Steven Mullaney suffered his first setback leading England Lions as his team were well beaten by India A at the Fischer County Ground, Grace Road.
In the tri-nation series that also includes the West Indies A, the Nottinghamshire all-rounder had captained the hosts to two comprehensive victories leading into this fixture.
But this encounter against the Indian visitors proved to be a different story with a second Mayank Agarwal century in two days setting the tourists on their way to a 102-run success.
After winning the toss and electing to bat first, opener Agarwal hit 112 in partnership with Shubman Gill (72) as the pair put on 165 for the first wicket before the former was second out to Mullaney.
Hanuma Vihari followed up with 69 and Deepak Hooda added a late 33 as India A accrued 309-6 off their 50 overs, with Matt Fisher taking 2-58 and Ed Barnard 2-51.
In reply, the Lions were always in trouble after being reduced to 32-3 following some fine bowling from Shardul Thakur (3-53).
Five England players got beyond 20 but none could go on to play an innings substantial enough to ignite a successful run chase as Liam Dawson (38) was the top scorer and Mullaney made 23, with the hosts all out for 207.
The Lions are next in action on Thursday when they take on the West Indies A for a second time at Northampton.
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